Alkaline protease DJP-100 is produced from Bacillus licheniformis through submerged fermentation, extraction and refining process. DJP-100 can catalyze the hydrolysis of proteins under alkaline conditions. It is suitable for food and brewing industry, and also applicable for leather processing, silk degumming and the production of detergents.
PRODUCT CHARACTERISTICS
- Declared Enzyme: Protease
- EC Number: EC 3.4.21.62
- Appearance: Pale Brown Liquid
- Activity: 100,000 U/ml (minimum)
- Specific Gravity: 1.15 – 1.28 g/ml
Effect Of pH: DJP-100 works at pH ranging from 6.0 to 12.0 with its optimum pH range from 10.0 to 11.0.
Effect Of Temperature: DJP-100 works at temperatures from 30 to 60°C. Its optimum temperature range from 45-55°C.
The recommended usage:
- The ratio of material to liquid 1:3-9, pH 8-10, temperature 50-60 °C, enzyme 0.5-1.5%, enzymatic hydrolysis for 2-6 hours.
- In the production of condiments, DJP-100 can increase the content of amino acids in condiments and improve the flavor.
- In the production of chondroitin, DJP-100 can help to increase the yield and purity. After alkali extraction of the raw material, add 0.2-0.5% DJP-100 at pH 8-10, temperature 40-50 °C, and keep enzymatic reaction for 4-8 hours.
- In the production of heparin sodium, DJP-100 can improve the molecular uniformity and product purity. After salt hydrolysis of the raw materials, add 2-4g DJP-100 to each small intestine, then hydrolyze at 45-60 °C and pH9-11 for 4-6 hours.
- In leather processing, DJP-100 can help to simplify and shorten the process, improve the quality of the finished product, increase the yield, and reduce the cost. The dosage for the soaking process is 0.02~0.1% (on mass of material); the dosage for the leather softening is 0.05~0.2%, and it acts for 3~6 hours at 35~40℃; dosage for the unhairing is 0.1~0.3%, and it acts for 12~20 hours at 20~35℃. The pH during process is controlled at 9~11.
- In silk degumming, DJP-100 can protect the silk from damage and hairiness, and make it more fluffy. After the raw materials are pretreated, the enzyme is added at a dosage level of 0.8~2.4% at pH 9~11, and it acts for 30~60 minutes at 40~50℃
- In detergents, DJP-100 can greatly improve the decontamination effect. The dosage rate is 0.2~1% on the mass of the detergent.
Packaging: 25kg, 30kg HDPE drums or 1125 kg IBC totes.
Storage: Avoid direct sunlight, high temperature and humidity during transportation. The storage room should be kept clean, cool and dry, preferably with air-conditioner.
Shelf-life: 12 months provided this product is stored at 0∼10°C in its original packaging, sealed and unopened, protected from direct sunlight.
DJP-100 is applied to the hydrolysis of animal and plant proteins in the food industry to produce peptides or amino acids to form protein hydrolysates with unique flavor and improve the nutritional value of proteins.
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