NP-100 is a food-grade protease produced from classical strains of Bacillus subtilis through submerged fermentation, extraction and refining process. NP-100 can catalyze the hydrolysis of proteins under neutral condition and generate peptide and amino acids. NP-100 has found its wide applications in food industry.
PRODUCT CHARACTERISTICS
- Declared Enzyme: Protease
- EC Number: EC 3.4.24.28
- Appearance: Black Brown Liquid
- Activity: 100,000 U/ml (minimum)
- Product pH: 4.0 to 6.0
- Specific Gravity: 1.15 – 1.28 g/ml
Effect Of pH: NP-100 works at pH ranging from 5.0 to 10.0 with its optimum pH range from 6.0 to 8.0 and optimal pH is 6.5.
Effect Of Temperature: NP-100 works at temperatures from 30 to 60°C. Its optimum temperature range from 45-55°C and an optimum temperature 50°C.
Recommended dosage:
- Beer production, recommended dosage: 0.1~0.5 kg/ton of raw material.
- Soy Sauce, recommended dosage: 0.1~0.5 kg/ton of raw material.
- Hydrolysis Of Animal Protein, recommended dosage: 0.5~1.5 kg/ton of raw material.
- Hydrolysis Of Plant Protein, recommended dosage: 0.5~1.5 kg/ton of raw material.
- Leather, recommended dosage: 0.8~1.6 kg/ton of raw material
Packaging: 25kg, 30kg HDPE drums or 1125 kg IBC totes.
Storage: Avoid direct sunlight, high temperature and humidity during transportation. The storage room should be kept clean, cool and dry, preferably with air-conditioner.
Shelf-life: 12 months provided this product is stored at 0∼10°C in its original packaging, sealed and unopened, protected from direct sunlight.



NP-100 can effectively hydrolyze animal and plant proteins, including meat, soybean, gelatin, casein, whey, and cereals used in beer production. The optimal concentration of the substrate is 10 to 25%.
NP-100 can help to increase α-amino acid content in beer
Use of NP-100 can partly replace Koji to maximize the utilization rate of the raw materials, and maintain the desirable flavor and quality of final products
NP-100 can be used to hydrolyze meat, fish, terrapin, pig pancreas, silkworm pupae, etc. to produce peptide and amino acids.
Adding NP-100 into soy milk or instant soy milk powder can considerably increase the yield of proteins and the solid content.
NP-100 can be used in the treatment of red blood cell to produce decolorized and soluble blood hydrolyte, which can be used in the production of calcium supplements, oral liquid nutritional supplements and food additives to enhance nutrition in food and beverages.
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