HT-800 is a low pH tolerated Heat-stable Alpha-Amylase produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. HT-800 is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
Product Characteristics:
- Declared Enzyme: Alpha-Amylase
- Systematic Name: EC 3.2.1.1, 1,4-α-D-glucan glucanohydrolase
- Activity: 80,000 U/ml (Minimum)
- Appearance: Sepia brown Liquid
- Product pH: 5.0 to 7.0
- Specific gravity: 1.10 to 1.25 g/ml
Dosage Guidelines: Exact dosage rate of HT-800 depends on the actual processing conditions such as starch substrate, liquefaction equipment, pH, temperature, processing time, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
Effect Of pH: HT-800 can work at pH ranging from 5.0 to 7.0 with its optimal pH range from 5.4 to 6.0. The exact pH optimum depends on the actual processing conditions, including temperature, substrate concentration and reaction time.
Effect Of Temperature: For interval liquefaction process, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction process, HT-800 demonstrates exceptional thermal stability at temperatures from 105 to 110°C and keeps on liquefying efficiently for 5 to 7 minutes.
Concentration Of Calcium: The presence of calcium increases the thermal stability of HT-800. This enzyme requires a very low calcium concentration of 20 to 50 ppm to ensure its enzyme stability.
Inhibitors: Copper, titanium, and cobalt ions are moderate inhibitors for HT-800, whilst Aluminum, lead and zinc ions act as strong inhibitors.
Packaging: HT-800 is packaged in 25kg, 30kg food-grade HDPE plastic drums or 1125 kg IBC totes. Customized packaging is available upon request.
Storage & Shelf-life: Typical shelf-life is six months if this product is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight. Its shelf-life will be eighteen months if it is stored properly at 0∼10°C. HT-800 has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, and should be avoided.
As a robust Alpha-Amylase with superior thermal stability, HT-800 has found its
wide application in the production of starch sugar.
Use of HT-800 can benefit the production of starch sugar as following:
- Superior stability with low pH
- Decrease viscosity of starch quickly
- Eliminate undesirable color substances and non-fermentable sugars
- Effective liquefaction of various starch materials, such as tapioca, corn, wheat, rice, etc.
- Superior performance without adding calcium ion under majority processing conditions
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