HGA-101PU is a synthetic enzyme between Glucoamylase and Pullulanase. HGA-101PU contains strong Glucoamylase enzyme and Pullulanase enzyme with the function of debranching starch.
Glucoamylase is produced from controlled fermentation of selected strains of Aspergillus niger, while Pullulanase is produced from highly heat-resistant strains of Bacillus licheniformis.
Glucoamylase hydrolyzes the α-1,4 glucosidic bonds of starch to release glucose.
Pullulanase can cut the α-1,6 glucoside bond in the branched chain of amylopectin and cut the entire branch structure to form amylose.
Therefore, HGA-101PU has a higher glucose conversion rate when saccharifying starch into glucose.
Product Features:
- Glucoamylase activity: 100,000 U/ml (minimum)
- Pullulanase activity: 800 BU/ml (minimum)
- Sensory description: Yellow-brown liquid
- pH: 3.0 to 5.0
- Density: 1.10 to 1.25 g/ml
Impact of Ph.D. on HGA-101PU:
- HGA-101PU is active in the pH range of 3.0-5.5.
- The optimal pH range is 4.0-4.5 with the optimal pH value being 4.2
Impact of Temperature on HGA-101PU:
- The effective temperature range for HGA-101PU is 30 to 65°C, normal temperature range is 55 to 65°C, with the optimum temperature at 60°C.
- HGA-101PU can be inactivated at temperatures above 65°C. Typically, HGA-101PU will be completely inactivated at 80°C in 5 minutes.
Instructions for use:
- The general dosage is from 0.35 – 0.6kg/ton of dry starch.
- Recommended optimal temperature is 58 to 62°C, pH 4.0 to 4.5.
- First, it is necessary to adjust the pH and temperature, then add HGA-101PU.
- Add HGA-101PU to starch solutions such as corn starch, wheat, barley, tapioca, rice, and potato, to convert starch into glucose.
- The amount used depends on the specific raw material and actual processing conditions. Several tests should be carried out to determine the optimal content.
- The saccharification process should take about 32-55 hours.
HGA-101PU is designed with the following advantages:
- The conversion rate of starch into glucose is high
- Maximum use of raw materials
- Suitable for glucose production with DX ≥ 96.5%
- Able to work with highly concentrated substrates
- Stable operation, no need to adjust dosage for each production batch
Packaging: 25kg, 30kg, or 1125kg IBC barrels.
Storage and shelf life:
- Or packed according to the customer’s request
- Normal shelf life is 6 months if stored below 25°C in sealed, unopened packaging, away from direct sunlight.
- The shelf life can be as long as 18 months provided that this product is stored properly at 0 ∼ 10 ° C.
- Prolonged storage and/or unfavorable conditions such as higher temperatures may result in higher dosage requirements and therefore require attention.


HGA-101PU is designed with the following advantages:
- The conversion rate of starch into glucose is high
- Maximum use of raw materials
- Suitable for glucose production with DX ≥ 96.5%
- Able to work with highly concentrated substrates
- Stable operation, no need to adjust dosage for each production batch
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